KMID : 0903519900330040343
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology 1990 Volume.33 No. 4 p.343 ~ p.348
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cis - Diamminedichloroplatinum(¥±) induces denaturation and conformational changes in pBR322 DNA
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Abstract
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E. coli LE392, transformed with CDDP-treated pBR322 DNA, was plated on ampicillin containing media. The number of colonies formed on ampicillin containing agar plate was reduced to undetectable level after trot the DNA with 13.3 ¥ìM CDDP. The CDDP- treated pBR322 DNA was susceptible to angle strand DNA specific Sl nuclease and it¢¥s migration pattern in agarose gel electrophoresis was changed. These results suggest, that CDDP adduction to pBR322 DNA resulted in denaturation of the double helix and changes in it¢¥s conformation which ultimately leads to the inactivation of the ampicillin resistant gene(Received November 25, 1990, Accepted December 22, 1990).
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KEYWORD
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